A novel method for glucose detection using dual-modal carbon dots for colorimetric and ratiometric fluorescence analysis

Chunling Yuan, Xiaotiao Yao, Yuanjin Xu, Xiu Qin, Rui Shi, Shiqi Cheng, Yilin Wang

Article ID: 2043
Vol 5, Issue 1, 2024
DOI: https://doi.org/10.54517/aas.v5i1.2043
Received: 05 March 2024; Accepted: 12 April 2024; Available online: 01 May 2024;
Issue release: 30 June 2024

VIEWS - 4152 (Abstract)

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Abstract

Iron-based nitrogen co-doped carbon dots (Fe, N-CDs) were synthesized from taro leaf biomass via a hydrothermal process using ammonium ferric sulfate dodecahydrate and urea. The synthesized Fe, N-CDs exhibited peroxidase-like activity and strong fluorescence at 450 nm. A dual-mode colorimetric/ratiometric fluorescence assay for hydrogen peroxide (H2O2) detection was developed using Fe, N-CDs and o-phenylenediamine (OPD) as probes. In the presence of H2O2, OPD was oxidized to 2,3-diaminophenazine (DAP), which is yellow and absorbs at 420 nm. Under 360 nm excitation, DAP emits fluorescence at 550 nm and quenches the fluorescence of Fe, N-CDs at 450 nm due to the internal fluorescence filtering effect. This enables the quantitative analysis of H2O2 using the absorbance at 420 nm (A420) and the fluorescence intensity ratio of DAP to Fe, N-CDs (I550/I450). Since glucose oxidase can convert glucose to H2O2, the assay was extended to glucose determination. At pH 5.4, 40 ℃, with 1.75 mmol/L OPD and a 25-minute reaction time, the method showed a linear relationship between the A420 and I550/I450 values and glucose concentration in the range of 1.0~100 μmol/L, with detection limits of 0.8 μmol/L (colorimetry) and 0.6 μmol/L (ratiometry). The method was validated for glucose detection in human serum.


Keywords

carbon point; colorimetry; ratio fluorescence; glucose; determination


References

1.

1.         Zhang J, Dai X, Song ZL, et al. One-pot enzyme- and indicator-free colorimetric sensing of glucose based on MnO2 nano-oxidizer. Sensors and Actuators B: Chemical. 2020; 304: 127304. doi: 10.1016/j.snb.2019.127304

2.

2.         Baek SH, Roh J, Park CY, et al. Cu-nanoflower decorated gold nanoparticles-graphene oxide nanofiber as electrochemical biosensor for glucose detection. Materials Science and Engineering: C. 2020; 107: 110273. doi: 10.1016/j.msec.2019.110273

3.

3.         Xie WQ, Gong YX, Yu KX. Rapid quantitative detection of glucose content in glucose injection by reaction headspace gas chromatography. J. Chromatogr. A. 2017; 1520143-1520146.

4.

4.         Ling Z, Xu P, Zhong Z, et al. Sensitive determination of glucose in Dulbecco’s modified Eagle medium by high-performance liquid chromatography with 1-phenyl-3-methyl-5-pyrazolone derivatization: application to gluconeogenesis studies. Biomedical Chromatography. 2015; 30(4): 601-605. doi: 10.1002/bmc.3589

5.

5.         Huang HP, Yue YF, Xu L, et al. Glucose Biosensor Based on Dy2(MoO4)3-AuNPs Composite Nanomaterial. Chem. J. Chinese Universities. 2017; 38(4): 554-560.

6.

6.         Xu L, Lin YQ, Chen X, et al. Electrodeposition of Platinum Nanoparticles on MgAl-layered Double Hydroxide Modified Indium Tin Oxide Electrode for Electrochemical Glucose Biosensor. Chem. J. Chinese Universities. 2016; 37(3): 442-447.

7.

7.         Liu T, Zhang S, Liu W, et al. Smartphone based platform for ratiometric fluorometric and colorimetric determination H2O2 and glucose. Sensors and Actuators B: Chemical. 2020; 305: 127524. doi: 10.1016/j.snb.2019.127524

8.

8.         Rashtbari S, Dehghan G, Amini M. An ultrasensitive label-free colorimetric biosensor for the detection of glucose based on glucose oxidase-like activity of nanolayered manganese-calcium oxide. Analytica Chimica Acta. 2020; 1110: 98-108. doi: 10.1016/j.aca.2020.03.021

9.

9.         Cheng X, Huang L, Yang X, et al. Rational design of a stable peroxidase mimic for colorimetric detection of H2O2 and glucose: A synergistic CeO2/Zeolite Y nanocomposite. Journal of Colloid and Interface Science. 2019; 535: 425-435. doi: 10.1016/j.jcis.2018.09.101

10.

10.      Lin T, Zhong L, Guo L, et al. Seeing diabetes: visual detection of glucose based on the intrinsic peroxidase-like activity of MoS2 nanosheets. Nanoscale. 2014; 6(20): 11856-11862. doi: 10.1039/c4nr03393k

11.

11.      Liu H, Hua Y, Cai Y, et al. Mineralizing gold-silver bimetals into hemin-melamine matrix: A nanocomposite nanozyme for visual colorimetric analysis of H2O2 and glucose. Analytica Chimica Acta. 2019; 1092: 57-65. doi: 10.1016/j.aca.2019.09.025

12.

12.      Yuan J, Cen Y, Kong XJ, et al. MnO2-Nanosheet-Modified Upconversion Nanosystem for Sensitive Turn-On Fluorescence Detection of H2O2and Glucose in Blood. ACS Applied Materials & Interfaces. 2015; 7(19): 10548-10555. doi: 10.1021/acsami.5b02188

13.

13.      Wang HB, Chen Y, Li N, et al. A fluorescent glucose bioassay based on the hydrogen peroxide-induced decomposition of a quencher system composed of MnO2 nanosheets and copper nanoclusters. Microchimica Acta. 2016; 184(2): 515-523. doi: 10.1007/s00604-016-2045-7

14.

14.      Xiao N, Liu SG, Mo S, et al. B,N-carbon dots-based ratiometric fluorescent and colorimetric dual-readout sensor for H2O2 and H2O2-involved metabolites detection using ZnFe2O4 magnetic microspheres as peroxidase mimics. Sensors and Actuators B: Chemical. 2018; 273: 1735-1743. doi: 10.1016/j.snb.2018.07.097

15.

15.      Zhang W, Li X, Xu X, et al. Pd nanoparticle-decorated graphitic C3N4nanosheets with bifunctional peroxidase mimicking and ON–OFF fluorescence enable naked-eye and fluorescent dual-readout sensing of glucose. Journal of Materials Chemistry B. 2019; 7(2): 233-239. doi: 10.1039/c8tb02110d

16.

16.      Wang C, Tan R, Li L, et al. Dual-modal Colorimetric and Fluorometric Method for Glucose Detection Using MnO2 Sheets and Carbon Quantum Dots. Chemical Research in Chinese Universities. 2019; 35(5): 767-774. doi: 10.1007/s40242-019-9130-5

17.

17.      Zhong QM, Huang XH, Qin QM, et al. Determination of Glucose Based on Carbon Quantum Dots as Peroxidase Mimetic Enzyme. Chinese J. Anal. Chem. 2018; 46(7): 1062-1068.

18.

18.      Geng X, Sun Y, Li Z, et al. Retrosynthesis of Tunable Fluorescent Carbon Dots for Precise Long‐Term Mitochondrial Tracking. Small. 2019; 15(48). doi: 10.1002/smll.201901517

19.

19.      Guo S, Sun Y, Geng X, et al. Intrinsic lysosomal targeting fluorescent carbon dots with ultrastability for long-term lysosome imaging. Journal of Materials Chemistry B. 2020; 8(4): 736-742. doi: 10.1039/c9tb02043h

20.

20.      Chen Y, Qin X, Yuan C, et al. Double responsive analysis of carbaryl pesticide based on carbon quantum dots and Au nanoparticles. Dyes and Pigments. 2020; 181: 108529. doi: 10.1016/j.dyepig.2020.108529

21.

21.      Su A, Wang D, Shu X, et al. Synthesis of Fluorescent Carbon Quantum Dots from Dried Lemon Peel for Determination of Carmine in Drinks. Chemical Research in Chinese Universities. 2018; 34(2): 164-168. doi: 10.1007/s40242-018-7286-z

22.

22.      Yuan C, Qin X, Xu Y, et al. High sensitivity detection of H2O2 and glucose based on carbon quantum dots-catalyzed 3,3′,5,5′-tetramethylbenzidine oxidation. Microchemical Journal. 2020; 159: 105365. doi: 10.1016/j.microc.2020.105365

23.

23.      Yadav PK, Singh VK, Chandra S, et al. Green Synthesis of Fluorescent Carbon Quantum Dots from Azadirachta indica Leaves and Their Peroxidase-Mimetic Activity for the Detection of H2O2 and Ascorbic Acid in Common Fresh Fruits. ACS Biomaterials Science & Engineering. 2018; 5(2): 623-632. doi: 10.1021/acsbiomaterials.8b01528

24.

24.      Chandra S, Singh VK, Yadav PK, et al. Mustard seeds derived fluorescent carbon quantum dots and their peroxidase-like activity for colorimetric detection of H2O2 and ascorbic acid in a real sample. Analytica Chimica Acta. 2019; 1054: 145-156. doi: 10.1016/j.aca.2018.12.024

25.

25.      Wang L, Liu Y, Yang Z, et al. A ratiometric fluorescence and colorimetric dual-mode assay for H2O2 and xanthine based on Fe, N co-doped carbon dots. Dyes and Pigments. 2020; 180: 108486. doi: 10.1016/j.dyepig.2020.108486

26.

26.      Zhuo S, Guan Y, Li H, et al. Facile fabrication of fluorescent Fe-doped carbon quantum dots for dopamine sensing and bioimaging application. The Analyst. 2019; 144(2): 656-662. doi: 10.1039/c8an01741g

27.

27.      Hu Y, Zhang L, Li X, et al. Green Preparation of S and N Co-Doped Carbon Dots from Water Chestnut and Onion as Well as Their Use as an Off–On Fluorescent Probe for the Quantification and Imaging of Coenzyme A. ACS Sustainable Chemistry & Engineering. 2017; 5(6): 4992-5000. doi: 10.1021/acssuschemeng.7b00393

28.

28.      Sun X, He J, Yang S, et al. Green synthesis of carbon dots originated from Lycii Fructus for effective fluorescent sensing of ferric ion and multicolor cell imaging. Journal of Photochemistry and Photobiology B: Biology. 2017; 175: 219-225. doi: 10.1016/j.jphotobiol.2017.08.035

29.

29.      Shen J, Shang S, Chen X, et al. Facile synthesis of fluorescence carbon dots from sweet potato for Fe3+ sensing and cell imaging. Materials Science and Engineering: C. 2017; 76: 856-864. doi: 10.1016/j.msec.2017.03.178

30.

30.      Gu D, Shang S, Yu Q, et al. Green synthesis of nitrogen-doped carbon dots from lotus root for Hg(II) ions detection and cell imaging. Applied Surface Science. 2016; 390: 38-42. doi: 10.1016/j.apsusc.2016.08.012

31.

31.      Wen X, Shi L, Wen G, et al. Green synthesis of carbon nanodots from cotton for multicolor imaging, patterning, and sensing. Sensors and Actuators B: Chemical. 2015; 221: 769-776. doi: 10.1016/j.snb.2015.07.019

32.

32.      Yang W, Huang T, Zhao M, et al. High peroxidase-like activity of iron and nitrogen co-doped carbon dots and its application in immunosorbent assay. Talanta. 2017; 164: 1-6. doi: 10.1016/j.talanta.2016.10.099

33.

33.      Fang A, Long Q, Wu Q, et al. Upconversion nanosensor for sensitive fluorescence detection of Sudan I–IV based on inner filter effect. Talanta. 2016; 148: 129-134. doi: 10.1016/j.talanta.2015.10.048

34.

34.      Qian P, Qin Y, Lyu Y, et al. A hierarchical cobalt/carbon nanotube hybrid nanocomplex-based ratiometric fluorescent nanosensor for ultrasensitive detection of hydrogen peroxide and glucose in human serum. Analytical and Bioanalytical Chemistry. 2019; 411(8): 1517-1524. doi: 10.1007/s00216-019-01573-z

35.

35.      Xing Z, Tian J, Asiri AM, et al. Two-dimensional hybrid mesoporous Fe2O3-graphene nanostructures: A highly active and reusable peroxidase mimetic toward rapid, highly sensitive optical detection of glucose. Biosensors and Bioelectronics. 2014; 52: 452-457. doi: 10.1016/j.bios.2013.09.029

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